The association of exclusive breastfeeding duration with DNA methylation of genes involved in innate immunity like Toll-like receptor-1 (TLR-1) gene in children and adolescents
- مجال مشروع البحث–الرئيسي
- العلوم الطبية والصحية
- مجال مشروع البحث–الثانوي
- العلوم الجينية
- المؤسسة العلمية
- الجامعة الهاشمية
- المحافظة
- الزرقاء
- قيمة الدعم
- JD14227.8
- سنة الدعم
- 2014
- حالة المشروع
- منتهي مع النشر
- البحث منشور
- Hatmal, M. M. M., Issa, N. N., Alshaer, W., Al-Ameer, H. J., Abuyaman, O., Tayyem, R., & Hijjawi, N. S. (2019). Association of Breastfeeding Duration with Susceptibility to Allergy, Influenza, and Methylation Status of TLR1 Gene. Medicina, 55(9), 535.
- ملخص عن مشروع البحث
- Background and Objectives: This study aimed to investigate the possible association between exclusive breastfeeding duration during early infancy and susceptibility to allergy and influenza in adulthood. Furthermore, we also investigated the association of breastfeeding duration with DNA methylation at two sites in the promoter of the toll-like receptor-1 (TLR1) gene, as well as the association between DNA methylation of the toll-like receptor-1 (TLR1) gene and susceptibility to different diseases. Materials and Methods: Blood samples were collected from 100 adults and classified into two groups according to breastfeeding duration (<6 months and ≥6 months) during infancy. Subjects were asked to complete a questionnaire on their susceptibilities to different diseases and sign a consent form separately. Fifty-three samples underwent DNA extraction, and the DNA samples were divided into two aliquots, one of which was treated with bisulfite reagent. The promoter region of the TLR1 gene was then amplified by polymerase chain reaction (PCR) and sequenced. Results: We found a significant association between increased breastfeeding duration and a reduction in susceptibility to influenza and allergy, as well asa significant reduction in DNA methylation within the promoter of the TLR1 gene. No association was found between DNA methylation and susceptibility to different diseases. Conclusions: The findings demonstrate the significance of increased breastfeeding duration for improved health outcomes at the gene level.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780093/
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الاسم الكامل للباحث الرئيسي
د.مأمون حتمل
الجنس
ذكر
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الباحث المشارك الأول
د. ريما تيم
الجنس
انثى
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الباحث المشارك الثاني
د.نوال حجاوي
الجنس
انثى
المشاريع ذات صلة