مؤسسة عبد الحميد شومان > البحث العلمي > صندوق دعم البحث العلمي

الرجوع

Gold Nanorods Loaded into Gels: Colloidal Stability, Rheological Properties, Photothermal Properties and Penetration extent into Excised Human Skin

مجال مشروع البحث–الرئيسي: العلوم الطبية والصحية
مجال مشروع البحث–الثانوي: العلوم الصيدلانية
المؤسسة العلمية: جامعة الزيتونة الاردنية
المحافظة: عمان
قيمة الدعم: JD14800
سنة الدعم: 2017
حالة المشروع: منتهي مع النشر
البحث منشور: (1) Mahmoud, N. N., Hikmat, S., Ghith, D. A., Hajeer, M., Hamadneh, L., Qattan, D., & Khalil, E. A. (2019). Gold nanoparticles loaded into polymeric hydrogel for wound healing in rats: Effect of nanoparticles’ shape and surface modification. International journal of pharmaceutics, 565, 174-186. (2) Mahmoud, N. N., Alhusban, A. A., Ali, J. I., Al-Bakri, A. G., Hamed, R., & Khalil, E. A. (2019). Preferential accumulation of phospholipid-PEG and cholesterol-PEG decorated gold nanorods into human skin layers and their photothermal-based antibacterial activity. Scientific reports, 9(1), 1-15. (3) Mahmoud, N. N., Hamed, R., & Khalil, E. A. (2020). Colloidal stability and rheological properties of gold nanoparticle–loaded polymeric hydrogels: impact of nanoparticle’s shape, surface modification, and concentration. Colloid and Polymer Science, 298(8), 989-999.
ملخص عن مشروع البحث: 1. Nanotechnology-based platforms have gained a growing interest in skin wound healing. Herein, gold nanoparticles (AuNPs) of different shapes (rods and spheres) and surface modifications (neutral, cationic and anionic charged polymers) were synthesized, characterized and loaded into a thermosensitive hydrogel (poloxamer 407). AuNPs-hydrogels exhibited excellent colloidal stability and demonstrated slow and prolonged release behavior over a 48-h of exposure using in vitro model. Hydrogels of poly ethylene glycol (PEG)-gold nanorods (AuNRs) and cationic poly allyl amine hydrochloride (PAH)-AuNRs demonstrated remarkable wound healing properties upon topical application on wounds using an animal model. PEGylated and cationic charged-AuNRs hydrogels have enhanced skin re-epithelization and collagen deposition after 14 days of daily wound treatment compared to controls, and they affected the gene expression of several inflammatory and anti-inflammatory mediators. Hydrogels of PEG-AuNRs and PAH-AuNRs exhibited potent in vitro antibacterial activity against staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). Furthermore, AuNPs of different shapes and surface modifications demonstrated low percentages of deposition into the main body organs after 21 days of daily wound treatment. Hydrogels of AuNRs could be a promising nano-platform for wound healing. https://www.sciencedirect.com/science/article/abs/pii/S0378517319303503 2. Herein, a library of gold nanorods (GNR) decorated with polyethylene glycol-thiol (PEG-SH) containing diferent functionalities were synthesized and characterized by optical absorption spectroscopy, zeta potential, dynamic light scattering (DLS), transmission electron microscope (TEM) and proton nuclear magnetic resonance (1H-NMR). The colloidal stability of GNR when exposed to skin, and their preferential accumulation into excised human skin layers were investigated. Confocal laser scanning microscopy, transmission electron microscope (TEM) and inductively coupled plasma_x0002_optical emission spectroscopy (ICP-OES) were utilized to track the penetration of GNR into diferent skin layers. The results demonstrated that cholesterol-PEG coated GNR were preferentially loaded up in the upper layers of skin (stratum corneum), while phospholipid-PEG coated counterparts were drastically deposited in skin dermis. Neutral methoxy-PEG-coated GNR were distributed in both SC and dermis skin layers, while charged GNR (anionic-carboxylic acid-PEG-GNR and cationic-amine-PEGGNR) revealed a minimal accumulation into skin. DSPE-PEG-GNR and Chol-PEG-GNR demonstrated antibacterial activities against Staphylococcus aureus (S aureus) at MIC values of 0.011nM and 0.75nM, respectively. Photothermal treatment for S. aureus at sub-MIC concentrations resulted in a signifcant bactericidal efect when using Chol-PEG-GNR but not DSPE-PEG-GNR. Gold-based nanoscale systemshave great value as a promising platform for skin diseases therapy. https://www.nature.com/articles/s41598-019-42047-7.pdf 3. Gold nanorods (GNR) and gold nanospheres (GNS) of different surface charges; neutral -poly ethylene glycol (PEG), negatively charged poly acrylic acid (PAA), and positively charged poly allyl amine hydrochloride (PAH), were synthesized and characterized, and their colloidal stability was investigated using UV-Vis absorption spectra and zeta potential measurements. Gold nanoparticles (GNP) were loaded at different concentrations (from 1.0 to 6.0 nM) into two hydrogels prepared in water; 18% w/v Poloxamer 407 and 1.5% w/v hydroxypropyl methylcellulose (HPMC). The GNP hydrogels were characterized in terms of GNP colloidal stability, and the rheological properties of the GNP hydrogels. GNP loaded into HPMC hydrogel demonstrated poor stability and exhibited separation into two layers. On the contrary, colloidal stability of the GNP was preserved upon loading into Poloxamer 407 hydrogel. The rheology study demonstrates that Poloxamer 407 hydrogel was able to accommodate GNR or GNS at concentrations lower than 6.0 nM with no apparent change in the measured rheology parameters. PAH-coated GNP caused the largest decrease in the low shear rate viscosity values. The elastic modulus was higher than the viscous modulus at the entire concentration range and the values of the phase shift angle did not exceed 10 degrees indicating prevail elastic property. As a conclusion, Poloxamer 407 hydrogel is a good candidate as a topical vehicle for both GNR and GNS at concentrations lower than 6.0 nM. https://link.springer.com/article/10.1007/s00396-020-04659-8